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Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis
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Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis

Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis

DOI:
10.3791/58626-v

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10:19 min

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January 07, 2019

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, , , , ,
1Division of Metabolism, Endocrinology and Lipid Research, Department of Medicine,Washington University School of Medicine, 2Department of Biomedical Engineering,Washington University in Saint Louis, 3Department of Ophthalmology and Visual Science,Washington University School of Medicine

Chapters

  • 00:04Title
  • 00:37Calibration of Extracellular Flux Instrument
  • 01:53Isolation of Fresh Mouse Retinal Tissue
  • 03:59Assay Protocol
  • 07:48Results: Energy Metabolism in the Transducin-Null Retina
  • 09:35Conclusion

Summary

Automatic Translation

Automatic Translation

This technique describes real time recording of oxygen consumption and extracellular acidification rates in explanted mouse retinal tissues using an extracellular flux analyzer.

Tags

Keywords: Extracellular Flux AnalysisEnergy MetabolismGlycolysisOxidative PhosphorylationMitochondrial StressMouse Retina

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