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Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
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Bioengineering
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JoVE Journal Bioengineering
Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

DOI:
10.3791/58634-v

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11:05 min

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October 25, 2018

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, , ,
1Physics Department,Technical University of Munich

Chapters

  • 00:04Title
  • 00:57Chip Fabrication
  • 02:43Substrate Preparation for Photolithography
  • 04:11Projection Lithography
  • 05:40Holder Assembly for Compartmentalized Gene Expression
  • 07:17Setup for Gene Expression Analysis
  • 09:23Results: Bephore Lithography and Compartmentalized Gene Expression
  • 10:29Conclusion

Summary

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We describe a simple lithographic procedure for the immobilization of gene-length DNA molecules on a surface, which can be used to perform cell-free gene expression experiments on biochips.

Tags

DNA BiochipsSelf Re-synthetic BiologyBephore LithographyDNA Strand DisplacementMultistep LithographyPEGylationBiotin-PEG-SilaneSilicon Wafer CleaningSubstrate PreparationGene ImmobilizationFunctional Gene BrushesProtein Expression

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