Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Engineering / Enrich and Expand Rare Antigen-specific T Cells…
JoVE Journal
Engineering

A subscription to JoVE is required to view this content.

JoVE Journal Engineering
Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles
3D Printing of Biomolecular Models for Research and Pedagogy
Journal (Engineering)
3D Printing of Biomolecular Models for Research and Pedagogy
A Method for Studying the Temperature Dependence of Dynamic Fracture and Fragmentation
Journal (Engineering)
A Method for Studying the Temperature Dependence of Dynamic Fracture and Fragmentation
 

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

Article DOI: 10.3791/58640-v • 09:28 min • November 17th, 2018
November 17th, 2018 •
1,2,3,4, 2,4
1Department of Biomedical Engineering, School of Medicine, Johns Hopkins University, 2Institute for Cell Engineering, School of Medicine, Johns Hopkins University, 3Institute for Nanobiotechnology, Johns Hopkins University, 4Department of Pathology, School of Medicine, Johns Hopkins University

Chapters

Summary
Automatic Translation

Antigen-specific T cells are difficult to characterize or utilize in therapies due to their extremely low frequency. Herein, we provide a protocol to develop a magnetic particle which can bind to antigen-specific T cells to enrich these cells and then to expand them several hundred-fold for both characterization and therapy.

Tags

Enrichment Expansion Rare Antigen-specific T Cells Magnetic Nanoparticles E Plus E Technique Immunotherapy Neoepitopes Personalized Epitopes Cancer Immunotherapy Antigen-specific Dynamics Cellular Therapy Nanoparticle Artificial Antigen-presenting Cell Off-the-shelf Product Individual Patient Immune Responses
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter