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Flow Cytometric Detection of Newly-formed Breast Cancer Stem Cell-like Cells After Apoptosis Reversal
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Cancer Research
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JoVE Journal Cancer Research
Flow Cytometric Detection of Newly-formed Breast Cancer Stem Cell-like Cells After Apoptosis Reversal

Flow Cytometric Detection of Newly-formed Breast Cancer Stem Cell-like Cells After Apoptosis Reversal

DOI:
10.3791/58642-v

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11:21 min

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January 26, 2019

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, , , ,
1School of Life Sciences,The Chinese University of Hong Kong, 2State Key Laboratory of Agrobiotechnology,The Chinese University of Hong Kong, 3Key Laboratory for Regenerative Medicine, Ministry of Education,The Chinese University of Hong Kong, 4Centre for Novel Biomaterials,The Chinese University of Hong Kong

Chapters

  • 00:04Title
  • 01:08Preparation of Breast Non-stem Cancer Cells
  • 05:08Apoptotic Induction and Detection
  • 07:17Isolation of Apoptotic Cells and Apoptosis Reversal Procedure
  • 08:22Measurement of Breast CSC-like Cells by Flow Cytometry
  • 09:11Results: CSC-like Cells Transited from Breast Non-stem Cancer Cells During Apoptosis Reversal
  • 10:29Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to isolate apoptotic breast cancer cells by fluorescence-activated cell sorting and further detect the transition of breast non-stem cancer cells to breast cancer stem cell-like cells after apoptosis reversal by flow cytometry.

Tags

Keywords: Flow CytometryBreast CancerStem CellsApoptosis ReversalCell IsolationCell CultureCell SortingCD44CD24Trypsin-EDTAFACS BufferFc Block

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