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Flow Cytometric Detection of Newly-formed Breast Cancer Stem Cell-like Cells After Apoptosis Reversal

Article DOI: 10.3791/58642-v • 11:21 min • January 26th, 2019
January 26th, 2019 •
1,2, 1, 1,2, 1,2, 1,2,3,4
1School of Life Sciences, The Chinese University of Hong Kong, 2State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, 3Key Laboratory for Regenerative Medicine, Ministry of Education, The Chinese University of Hong Kong, 4Centre for Novel Biomaterials, The Chinese University of Hong Kong

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Summary
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Here, we present a protocol to isolate apoptotic breast cancer cells by fluorescence-activated cell sorting and further detect the transition of breast non-stem cancer cells to breast cancer stem cell-like cells after apoptosis reversal by flow cytometry.

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Flow Cytometric Detection Breast Cancer Stem Cells Apoptosis Reversal Purification Technique Widely Available Materials And Equipment Isolation Of Cells Chemo Cells Normal Cells Apoptotic Stimuli Recovery Rate Starting Cell Number MCF-7 Cells MDA-MB-231 Cells T47D Cells
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