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Immunology and Infection

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JoVE Journal Immunology and Infection
Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses
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Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses

Article DOI: 10.3791/58654-v • 05:33 min • January 7th, 2019
January 7th, 2019 •
*1, *2, 1, 1, 1, 3,4, 1, 2
1Pathology Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, 2Department of Experimental Oncology, European Institute of Oncology, 3Gastroenterology and Endoscopy Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, 4Department of Pathophysiology and Transplantation, Università degli Studi di Milano
* These authors contributed equally

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Summary
Automatic Translation

Lack of standardization for murine tissue processing reduces the quality of murine histopathological analysis as compared to human specimens. Here, we present a protocol to perform histopathological examination of murine inflamed and uninflamed colonic tissues to show the feasibility of robotic systems routinely used for processing and embedding human samples.

Tags

Robotic Systems Colonic Murine Samples Histological Analyses Automated Protocol Histopathological Preparation Procedural Steps Francesca Boggio Pathologist Fixed Murine Colon Tissue Samples Neutral Buffered Formalin Sectioning Work Plate Petri Dish Sterile Scalpel Centimeter Fragments Oriented Paraffin-embedding Cassette Plastic Supporting Frame Labeling Automated Processor Paraffin Melting Metal Basket
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