Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages <em>In Vitro</em> and <em>In Vivo</em>

Na Jia; Haoan Wu; Juanli Duan; Chunxue Wei; Kaixuan Wang; Yu Zhang&#160;; Xiaohua Mao

doi:10.3791/58660

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfer...

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Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

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09:36 min

February 5, 2019

DOI: 10.3791/58660-v

Na Jia*¹, Haoan Wu*², Juanli Duan¹, Chunxue Wei¹, Kaixuan Wang², Yu Zhang ², Xiaohua Mao¹

¹Key Laboratory of Ministry of Education for Developmental Genes & Human Diseases, Institute of Life Sciences and School of Medicine,Southeast University, ²State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials & Devices, School of Biological Science & Medical Engineering,Southeast University

We describe a method of using polyethyleneimine (PEI)-coated superparamagnetic iron oxide nanoparticles for transfecting macrophages with siRNA. These nanoparticles can efficiently deliver siRNA to macrophages in vitro and in vivo and silence target gene expression.

This measure can help answer key questions. In the field of humanology. That answer in the function of macrophages under physiological end to pathological conditions.

The main advantage of this technique is that polyethyleneimine coated can efficiently en vitro without compromising cell. Under optimal doses. The implications of this technique extend toward therapy and diagnosis of many chronic inflammatory disorders and cancers.

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