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JoVE Journal Environment
An Optimized Rhizobox Protocol to Visualize Root Growth and Responsiveness to Localized Nutrients
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An Optimized Rhizobox Protocol to Visualize Root Growth and Responsiveness to Localized Nutrients

Article DOI: 10.3791/58674-v • 07:45 min • October 22nd, 2018
October 22nd, 2018 •
1, 2, 1
1Department of Plant Sciences, University of California at Davis, 2Department of Natural Resources and the Environment, University of New Hampshire

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Summary
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Visualizing and measuring root growth in situ is extremely challenging. We present a customizable rhizobox method to track root development and proliferation over time in response to nutrient enrichment. This method is used to analyze maize genotypic differences in root plasticity in response to an organic nitrogen source.

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Optimized Rhizobox Protocol Visualize Root Growth Responsiveness To Localized Nutrients Root And Rhizosphere Science Non-invasive Measurements Single Root System Soil Specialized Equipment Relatively Inexpensive Rhizobox Assembly Clear Acrylic Panels Side And Bottom Spacers Polyester Padding Prevent Leaking Hardware Assembly Screw Washers Hex Nut Treatment And Control Patches High Density Polyethylene Sheets Light Deprivation Heat Reduction
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