Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture

Journal

Biology

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture

DOI:

10.3791/58701-v

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06:16 min

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August 10, 2019

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Rebecca J. Morris, Nyssa Readio, Kelsey Boland, Kelly Johnson, Sonali Lad, Anupama Singh, Ashok Singh, Stephanie Holtorf, Samantha Skaar

¹Hormel Institute,University of Minnesota

Chapters

00:04Title
00:30Primary Keratinocyte Harvesting and Seeding
05:09Results: Representative Mouse Epidermal Keratinocyte Clonogenic Culture Characteristics
05:43Conclusion

Summary

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The goal of this protocol is to isolate epidermal keratinocytes from the dorsal skin of adult mice for a variety of downstream applications such as molecular biology, biochemistry, fluorescence activated cell sorting, and primary in vitro uses (e.g., clonogenic keratinocytes).

Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture

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