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Journal / Biology / Transcriptomics de célula única baseada em código …
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Biology

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JoVE Journal Biology
Transcriptomics de célula única baseada em código de barras da gota de tecidos de mamíferos adultos
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Transcriptomics de célula única baseada em código de barras da gota de tecidos de mamíferos adultos

Article DOI: 10.3791/58709-v • 10:12 min • January 10th, 2019
January 10th, 2019 •
*1,2,3, *2, 2, 2, 1,4, 2, 1,4, 1,2,3
1Hotchkiss Brain Institute, University of Calgary, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, 3Alberta Children's Hospital Research Institute, University of Calgary, 4Department of Clinical Neurosciences, Cumming School of Medicine, University of Calgary
* These authors contributed equally

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Este protocolo descreve os processos gerais e controle de qualidade verifica necessário para a preparação de células simples mamíferos adultas saudáveis para preparações de RNA-Seq de célula única baseada em gotículas, alta taxa de transferência. Parâmetros de sequenciamento, alinhamento de leitura e análise de bioinformatic a jusante de célula única também são fornecidos.

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Biologia questão 143 única célula o RNA-Seq adulto transcriptoma RNA FACS
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