Journal
/
Immunology and Infection
/
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

DOI:
10.3791/58711-v

•

07:52 min

•

January 09, 2019

•

, , , , , , , , ,
1Department of Laboratory Medicine,AnSteel Group General Hospital, 2Cancer Science Institute of Singapore,National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine,National University of Singapore, 4Department of Haematology-Oncology,National University Health System

Chapters

  • 00:04Title
  • 00:57Monoplex Tyramide-Based Immunofluorescence
  • 03:22Monoplex Slide and Tissue Microarray (TMA) Scanning
  • 04:36Data Analysis
  • 06:28Results: Representative Mutiplexed Fluorescent Immunohistochemical (mf-IHC) Staining, Imaging, and Analysis
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a protocol for multiplex fluorescent immunohistochemical staining and imaging for the simultaneous localization of multiple cancer-associated antigens in lymphoma. This protocol can be extended to the colocalization analysis of biomarkers within all tissue sections.

Tags

Keywords: Multiplexed Fluorescent ImmunohistochemistryLymphomaTumor MicroenvironmentAntigen Co-expressionSpatial RelationshipAntigen RetrievalMicrowave StrippingPeroxidase BlockTyramide-based Fluorescent ReagentSpectral Imaging

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58711?article_type=v

Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice
check_url/58711?article_type=v

Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples
check_url/58711?article_type=v

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
check_url/58711?article_type=v

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
check_url/58711?article_type=v

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
check_url/58711?article_type=v

Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
check_url/58711?article_type=v

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
check_url/58711?article_type=v

Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment
check_url/58711?article_type=v

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
check_url/58711?article_type=v

Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses

Read Article