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JoVE Journal
Immunology and Infection

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Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro
 

Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro

Article doi: 10.3791/58759
November 26th, 2018

Summary November 26th, 2018

Marginal zone B cells (MZBs) respond to the force of shear flow by re-orienting their migration path up the flow. This protocol shows how to record and analyze the migration using a fluidics unit, pump, microscope imaging system, and free software.

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