This content is Free Access.
Chapters
- 00:04Title
- 00:27Identification and Cloning of Guide RNA Sequence
- 04:28Designing and Generation of Repair Template
- 05:33Transformation of C. albicans with Repair Template and Plasmid
- 07:47Colony PCR
- 08:52Results: Evaluation of Successful Genome Editing in C. albicans
- 09:27Conclusion
Efficient genome engineering of Candida albicans is critical to understanding the pathogenesis and development of therapeutics. Here, we described a protocol to quickly and accurately edit the C. albicans genome using CRISPR. The protocol allows investigators to introduce a wide variety of genetic modifications including point mutations, insertions, and deletions.
