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Evaluering af virulens og patogenese af Aeromonas infektion i en Caenorhabditis elegans Model
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Immunology and Infection
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JoVE Journal Immunology and Infection
Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model

Evaluering af virulens og patogenese af Aeromonas infektion i en Caenorhabditis elegans Model

DOI:
10.3791/58768-v

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06:15 min

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December 20, 2018

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, , ,
1Institute of Basic Medical Sciences, College of Medicine,National Cheng Kung University, 2Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine,National Cheng Kung University, 3Department of Biochemistry and Molecular Biology, College of Medicine,National Cheng Kung University, 4Department of Microbiology and Immunology, College of Medicine,National Cheng Kung University

Chapters

  • 00:04Title
  • 00:37Synchronization of C. elegans
  • 02:24C. elegans Survival Assay with Aeromonas
  • 03:22C. elegans Liquid Toxicity Assay with Aeromonas
  • 04:14C. elegans Muscle Necrosis Assay with Aeromonas
  • 04:57Results: Diversity in Survival Rates of C. elegans Infected with Aeromonas spp. Consistent with Clinical Observations
  • 05:45Conclusion

Summary

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Automatic Translation

Her, vil vi præsentere tre forskellige eksperimenter for at studere Aeromonas infektion i C. elegans. Ved hjælp af disse praktiske metoder, er det let at vurdere toksicitet mellem og inden for Aeromonas arter.

Tags

AeromonasCaenorhabditis ElegansVirulencePathogenesisHost-pathogen InteractionToxicityPathogenicityL1 WormsL4 WormsNGM PlatesE. Coli OP50Bacterial BrothOD 600 Absorbance

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