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Chapters
- 00:04Title
- 00:48Dissection and Isolation of Cerebral Artery
- 02:34Preparation of Endothelial Tube and Superfusion
- 05:44Simultaneous Measurement of [Ca2+]i and Vm
- 08:36Results: Simultaneous [Ca2+]i and Vm Recordings
- 09:10Conclusion
Vist her er protokoller for (1) frisk isolere intakt cerebral endotel "rør" og (2) samtidige målinger i endothelial calcium og membran potentiale under endotel-afledte hyperpolarisering. Yderligere, disse metoder mulighed for farmakologiske tuning i endothelial celle calcium og elektriske signaler som enkelte eller interaktive eksperimentel variable.
