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JoVE Journal
Immunology and Infection

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Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
 

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

Article doi: 10.3791/58844
January 9th, 2019

Summary January 9th, 2019

This article illustrates a powerful method to quantify mitochondria or lysosomes in living cells. The combination of lysosome- or mitochondria-specific dyes with fluorescently conjugated antibodies against surface markers allows the quantification of these organelles in mixed cell populations, like primary cells harvested from tissue samples, by using multicolor flow cytometry.

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