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Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
 

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Article doi: 10.3791/58933
February 15th, 2019

Summary February 15th, 2019

The goal of this protocol is to isolate nonhuman primate CD34+ cells from primed bone marrow, to gene-modify these cells with lentiviral vectors, and to prepare a product for infusion into the autologous host. The total protocol length is approximately 48 h.

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