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Cancer Research

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Detection of Protease Activity by Fluorescent Peptide Zymography
 

Detection of Protease Activity by Fluorescent Peptide Zymography

Article DOI: 10.3791/58938-v
January 20th, 2019

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Summary January 20th, 2019

Here, we present a detailed protocol for a modified zymographic technique in which fluorescent peptides are used as the degradable substrate in place of native proteins. Electrophoresis of biological samples in fluorescent peptide zymograms enables detection of a wider range of proteases than previous zymographic techniques.

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