Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.

Detection of Protease Activity by Fluorescent Peptide Zymography
 

Detection of Protease Activity by Fluorescent Peptide Zymography

Article doi: 10.3791/58938
January 20th, 2019

Summary January 20th, 2019

Here, we present a detailed protocol for a modified zymographic technique in which fluorescent peptides are used as the degradable substrate in place of native proteins. Electrophoresis of biological samples in fluorescent peptide zymograms enables detection of a wider range of proteases than previous zymographic techniques.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter