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Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

DOI:
10.3791/58946-v

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08:49 min

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January 22, 2019

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, , , ,
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 2Singapore Immunology Network,A*STAR, 3Curiox Biosystems, 4Department of Immunobiology, Rega Institute for Medical Research,Katholieke Universiteit (KU) Leuven

Chapters

  • 00:04Title
  • 01:15Kinase Inhibitor Library Screening (Centrifuge-independent Assay)
  • 02:15Stimulation of Thymocytes Using Anti-CD3/CD28 Beads
  • 03:52Staining of Surface Antigens and Fixation of Cells
  • 05:09Intracellular Staining for Active Caspase 3
  • 06:05Results: Flow-Cytometry-Based Screening of Modulators of T-cell Receptor Signaling and T-cell Activation
  • 07:59Conclusion

Summary

Automatic Translation

Automatic Translation

This paper uses a flow-cytometry-based assay to screen libraries of chemical inhibitors for the identification of inhibitors and their targets that influence T-cell receptor signaling. The methods described here can also be expanded for high-throughput screenings.

Tags

Keywords: T-cell Receptor SignalingChemical Inhibitor LibrariesHigh-throughput AssayTCR Signaling MediatorsImmune DiseasesThymocytesKinase InhibitorsAnti-CD3/CD28 BeadsApoptosis

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