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An Induction System for Clustered Stomata by Sugar Solution Immersion Treatment in Arabidopsis thaliana Seedlings
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An Induction System for Clustered Stomata by Sugar Solution Immersion Treatment in Arabidopsis thaliana Seedlings

An Induction System for Clustered Stomata by Sugar Solution Immersion Treatment in Arabidopsis thaliana Seedlings

DOI:
10.3791/58951-v

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04:32 min

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February 15, 2019

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,
1Department of Integrated Frontier Sciences,The University of Tokyo, 2International Research Organization for Advanced Science and Technology,Kumamoto University

Chapters

  • 00:04Title
  • 00:31Preparation of 3% Sucrose Containing 1/2 Murashige-Skoog Medium Solution
  • 01:12Induction of Clustered Stomata by Sucrose-containing Medium Solution Immersion Treatment
  • 02:30Microscopic Observation of Clustered Stomata
  • 03:15Stomata Clustering Induced in A. thaliana Seedlings
  • 04:06Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to demonstrate how to induce clustered stomata in cotyledons of Arabidopsis thaliana seedlings by immersion treatment with a sugar-containing medium solution and how to observe intracellular structures such as chloroplasts and microtubules in the clustered guard cells using confocal laser microscopy.

Tags

Keywords: Arabidopsis ThalianaStomataSucroseInduction SystemClustered StomataMurashige-Skoog MediumConfocal Laser MicroscopyChloroplastsPlant Development

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