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Developmental Biology

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Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
 

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Article DOI: 10.3791/58975-v
June 11th, 2019

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Summary June 11th, 2019

This protocol details a monolayer, serum-free method to efficiently generate hepatocyte-like cells from human pluripotent stem cells (hPSCs) in 18 days. This entails six steps as hPSCs sequentially differentiate into intermediate cell-types such as the primitive streak, definitive endoderm, posterior foregut and liver bud progenitors before forming hepatocyte-like cells.

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