Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Immobilization of Live Caenorhabditis elegans…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.

JoVE Journal Bioengineering
Immobilization of Live Caenorhabditis elegans Individuals Using an Ultra-thin Polydimethylsiloxane Microfluidic Chip with Water Retention
Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology
Journal (Bioengineering)
Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology
A Microfluidic Technique to Probe Cell Deformability
Journal (Bioengineering)
A Microfluidic Technique to Probe Cell Deformability
 

Immobilization of Live Caenorhabditis elegans Individuals Using an Ultra-thin Polydimethylsiloxane Microfluidic Chip with Water Retention

Article DOI: 10.3791/59008-v • 04:55 min • March 19th, 2019
March 19th, 2019 •
1, 1, 1
1Department of Radiation-Applied Biology Research, Takasaki Advanced Radiation Research Institute, National Institutes for Quantum and Radiological Science and Technology (QST-Takasaki)

Chapters

Summary
Automatic Translation

A series of immobilization methods has been established to allow the targeted irradiation of live Caenorhabditis elegans individuals using a recently developed ultra-thin polydimethylsiloxane microfluidic chip with water retention. This novel on-chip immobilization is also adequate for imaging observations. The detailed treatment and application examples of the chip are explained.

Tags

Caenorhabditis Elegans Immobilization Microfluidic Chip Water Retention Worm Sheet Drying Prevention Microbeam Irradiation Behavioral Assays Long-term Observation Economical Transparent Sheet Bottom Cover Film Buffer Solution Platina Picker
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter