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Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles
This content is Free Access.
Chapters
- 00:04Title
- 00:37Shed Skin Extraction and Lipid Mass Determination
- 03:01Chromatography Column Preparation
- 05:19Lipid Extract Fractionation
- 06:29Results: Representative Standardized Fraction Masses Following Elution
- 07:23Conclusion
In reptiles, skin lipids from conspecifics are crucial for sexual signaling, with potential use in invasive species management. Here, we describe protocols for extracting skin lipids from shed skin or whole animals, determining and analyzing the total lipid mass, and separating the lipids using fractionation via column chromatography.
