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Journal / Biochemistry / Celle fraktionering af U937 celler i mangel af…
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Biochemistry

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JoVE Journal Biochemistry
Celle fraktionering af U937 celler i mangel af højhastighedstog centrifugering
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Celle fraktionering af U937 celler i mangel af højhastighedstog centrifugering

Article DOI: 10.3791/59022-v • 06:53 min • January 18th, 2019
January 18th, 2019 •
1, 1, 1, 1, 1, 1
1Department of Basic and Clinical Sciences, Albany College of Pharmacy and Health Sciences

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Vi præsenterer her, en protokol for at isolere plasma membran, cytoplasma og mitokondrier af U937 celler uden brug af højhastighedstog centrifugering. Denne teknik kan bruges til at rense subcellulært brøker for efterfølgende undersøgelse af protein lokalisering via immunoblotting.

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Biokemi spørgsmålet 143 cellulære fraktionering mitokondrier membran cytoplasma organeller centrifugering U937
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