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Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
DOI:

06:23 min

February 15, 2019

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Chapters

  • 00:04Title
  • 00:36System Inoculation
  • 01:38Luminal Fluid and Mucosal Sample Harvest
  • 03:30Daily Feeding Cycles
  • 04:27Results: Representative Stable Community Comparison Between the In Vitro System and the Fecal Inoculum
  • 05:52Conclusion

Summary

Automatic Translation

Here, we present a protocol for culturing the gut microbiota of the colon in vitro, using a series of bioreactors that simulate the physiological conditions of the gastro intestinal tract.

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