Journal
/
Bioengineering
/
Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota

DOI:
10.3791/59054-v

•

06:23 min

•

February 15, 2019

•

, , , , ,
1Dairy and Functional Foods Research Unit, Eastern Regional Research Center,Agricultural Research Service, United States Department of Agriculture, 2ProDigest, 3Division of Gastroenterology, Hepatology, and Nutrition,Children’s Hospital of Philadelphia

Chapters

  • 00:04Title
  • 00:36System Inoculation
  • 01:38Luminal Fluid and Mucosal Sample Harvest
  • 03:30Daily Feeding Cycles
  • 04:27Results: Representative Stable Community Comparison Between the In Vitro System and the Fecal Inoculum
  • 05:52Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for culturing the gut microbiota of the colon in vitro, using a series of bioreactors that simulate the physiological conditions of the gastro intestinal tract.

Tags

In Vitro CulturingGut MicrobiotaColonDefined MediumMucinFecal HomogenateBioreactorPH ControlLuminal SampleMucosal SampleDNA AnalysisShort-chain Fatty Acids

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology

Culturing Mouse Cardiac Valves in the Miniature Tissue Culture System

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model

TAPE: A Biodegradable Hemostatic Glue Inspired by a Ubiquitous Compound in Plants for Surgical Application

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

Melt Electrospinning Writing of Three-dimensional Poly(ε-caprolactone) Scaffolds with Controllable Morphologies for Tissue Engineering Applications

Creating a Structurally Realistic Finite Element Geometric Model of a Cardiomyocyte to Study the Role of Cellular Architecture in Cardiomyocyte Systems Biology

Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro

Read Article