Presented here are four protocols to construct and exploit yeast Saccharomyces cerevisiae reporter strains to study human P53 transactivation potential, impacts of its various cancer-associated mutations, co-expressed interacting proteins, and the effects of specific small molecules.
Monti, P., Bosco, B., Gomes, S., Saraiva, L., Fronza, G., Inga, A. Yeast As a Chassis for Developing Functional Assays to Study Human P53. J. Vis. Exp. (150), e59071, doi:10.3791/59071 (2019).