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Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery
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Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery

Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery

English

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6,566 Views

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06:32 min

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July 02, 2019

DOI:

10.3791/59072-v

DOI:
10.3791/59072-v

•

06:32 min
July 02, 2019

•

1 Views
, ,
1Department of Pharmacology and Toxicology,University of Mississippi Medical Center

Summary

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The primary goal of this article is to provide details of how to record membrane potential (Vm) from the middle cerebral artery using the microelectrode impalement method. The cannulated middle cerebral artery is equilibrated to gain myogenic tone, and the vessel wall is impaled using high resistance microelectrodes.

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Cite this Article

Reed, J. T., Sontakke, S. P., Pabbidi, M. R. Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery. J. Vis. Exp. (149), e59072, doi:10.3791/59072 (2019).

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