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JoVE Journal
Biochemistry

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Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
 

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Article doi: 10.3791/59073
February 13th, 2019

Summary February 13th, 2019

Fluorescence resonance energy transfer-based real-time observation systems of the DNA strand exchange reaction mediated by Rad51 were developed. Using the protocols presented here, we are able to detect the formation of reaction intermediates and their conversion into products, while also analyzing the enzymatic kinetics of the reaction.

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