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Biochemistry

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Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
 

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Article doi: 10.3791/59079
March 23rd, 2020

Summary March 23rd, 2020

We present a method for the purification, detection, and identification of diGly peptides that originate from ubiquitinated proteins from complex biological samples. The presented method is reproducible, robust, and outperforms published methods with respect to the level of depth of the ubiquitinome analysis.

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