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JoVE Journal
Biochemistry

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Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
 

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Article DOI: 10.3791/59079-v 11:54 min March 23rd, 2020
March 23rd, 2020

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Summary

We present a method for the purification, detection, and identification of diGly peptides that originate from ubiquitinated proteins from complex biological samples. The presented method is reproducible, robust, and outperforms published methods with respect to the level of depth of the ubiquitinome analysis.

Tags

Keywords: Protein Ubiquitination DiGly Peptides Mass Spectrometry SILAC Protein Enrichment Lys-C Trypsin Digestion TFA Precipitation
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