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JoVE Journal Biochemistry
Påvisning af proteinubiquitinationssteder ved peptidberigelse og massespektrometri
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Påvisning af proteinubiquitinationssteder ved peptidberigelse og massespektrometri

Article DOI: 10.3791/59079-v • 11:54 min • March 23rd, 2020
March 23rd, 2020 •
1, 1, 1
1Proteomics Center, Erasmus University Medical Center

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Vi præsenterer en metode til rensning, påvisning og identifikation af diGly peptider, der stammer fra ubiquitinated proteiner fra komplekse biologiske prøver. Den præsenterede metode er reproducerbar, robust og overgår offentliggjorte metoder med hensyn til dybden af ubiquitinomanalysen.

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Biokemi allestedsnærværelse ubiquitin ubiquitinome posttranslationel modifikation (PTM) diGly peptid immunrensning orbitrap massespektrometri
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