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Genetics

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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
 

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Article doi: 10.3791/59086
April 11th, 2019

Summary April 11th, 2019

CRISPR-Cas is a powerful technology to engineer the complex genomes of plants and animals. Here, we detail a protocol to efficiently edit the human genome using different Cas endonucleases. We highlight important considerations and design parameters to optimize editing efficiency.

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