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Journal / Genetics / Genomet redigering i pattedyr linjer med…
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Genomet redigering i pattedyr linjer med CRISPR-Cas
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Genomet redigering i pattedyr linjer med CRISPR-Cas

Article DOI: 10.3791/59086-v • 07:56 min • April 11th, 2019
April 11th, 2019 •
1, 1, 1,2, 1,2
1Genome Institute of Singapore, Agency for Science Technology and Research, 2School of Chemical and Biomedical Engineering, Nanyang Technological University

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CRISPR-Cas er en kraftig teknologi til ingeniør komplekse genomer av planter og dyr. Her, detalj vi en protokoll effektivt redigere det menneskelige genomet bruker forskjellige Cas-endonucleases. Vi markere viktige hensyn og design parametere for å optimalisere redigering effektivitet.

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Genetikk problemet 146 CRISPR Cas9 Cas12a genomet redigering ikke-homologe slutten begynte homologi-rettet reparasjon
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