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Chapters
- 00:04Title
- 00:40Fibrovascular Tissue Dissection
- 01:37Casting Upright Fibrin Gel Droplets for the Characterization of Native FT and Ex Vivo Culture
- 02:52Native FT and Ex Vivo Culture End-Point
- 03:19Whole-Mount Immunofluorescence Staining
- 07:00Results: PDR Ex Vivo Culture Induced Sprouting of CD31-positive Endothelium and Vasculature Preservation in Response to VEGFA
- 07:31Conclusion
Here, we present a protocol to study the pathophysiology of proliferative diabetic retinopathy by using patient-derived, surgically-excised, fibrovascular tissues for three-dimensional native tissue characterization and ex vivo culture. This ex vivo culture model is also amenable for testing or developing new treatments.
