Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells

Emma Pip&#243;-Oll&#233;; Prasad Walke; Martine K. Notabi; Rime B. El-Houri; Morten &#216;stergaard Andersen; David Needham; Eva C. Arnspang

doi:10.3791/59094

Journal

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Bioengineering

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Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells

8,947 Views

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09:34 min

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February 09, 2019

DOI:

10.3791/59094-v

DOI:

10.3791/59094-v

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09:34 min

February 09, 2019

•

8904 Views

Emma Pipó-Ollé¹, Prasad Walke², Martine K. Notabi¹, Rime B. El-Houri¹, Morten Østergaard Andersen¹, David Needham^2,3,4, Eva C. Arnspang¹

¹Department of Chemical Engineering, Biotechnology and Environmental Technology,University of Southern Denmark ²Center for Single Particle Science and Engineering (SPSE), Institute for Molecular Medicine, Health Sciences,University of Southern Denmark ³Department of Mechanical Engineering and Material Science,Duke University ⁴School of Pharmacy,University of Nottingham

Summary

This article describes the encapsulation of falcarindiol in lipid-coated 74 nm nanoparticles. The cellular uptake of the nanoparticles by human stem cells into lipid droplets is monitored by fluorescent and confocal imaging. Nanoparticles are fabricated by the rapid injection method of solvent shifting, and their size is measured with the dynamic light scattering technique.