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Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method

Article DOI: 10.3791/59112-v • 06:06 min • June 21st, 2019
June 21st, 2019 •
*1, *1, 1
1Department of Molecular Biology and Biochemistry, Simon Fraser University
* These authors contributed equally

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Summary
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Here we present a sensitive, rapid, and discriminating post-gel staining method to image RNAs tagged with RNA Mango aptamers I, II, III, or IV, using either native or denaturing polyacrylamide gel electrophoresis (PAGE) gels. After running standard PAGE gels, Mango-tagged RNA can be easily stained with TO1-Biotin and then analyzed using commonly available fluorescence readers.

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Fluorescent Visualization Mango-tagged RNA Polyacrylamide Gels Poststaining Method Sensitivity Specificity Purification Biologically Important RNAs RNA Complexes Diseases Tracking Visualization Denaturing Gel Bromophenol Blue Xylene Cyanol Dyes Band Separation Gel Casting Apparatus Polymerization Gel Tank Binder Clips Sample Loading RNA Samples
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