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Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method
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Biochemistry
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JoVE Journal Biochemistry
Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method

Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method

DOI:
10.3791/59112-v

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06:06 min

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June 21, 2019

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, ,
1Department of Molecular Biology and Biochemistry,Simon Fraser University

Chapters

  • 00:04Title
  • 00:41Preparing and Loading Denaturing Gels
  • 02:30Post Gel Staining
  • 03:17Imaging Mango Tagged RNAs in Gel
  • 04:01Results: Post-gel Staining Method to Image RNAs Tagged with RNA Mango Aptamers
  • 05:37Conclusion

Summary

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Here we present a sensitive, rapid, and discriminating post-gel staining method to image RNAs tagged with RNA Mango aptamers I, II, III, or IV, using either native or denaturing polyacrylamide gel electrophoresis (PAGE) gels. After running standard PAGE gels, Mango-tagged RNA can be easily stained with TO1-Biotin and then analyzed using commonly available fluorescence readers.

Tags

Keywords: Mango-tagged RNAPolyacrylamide GelsPoststaining MethodFluorescent VisualizationRNA PurificationRNA TrackingRNA VisualizationDenaturing GelGel ElectrophoresisRNA SamplesGel Staining SolutionGel Imaging

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