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Developmental Biology

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Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone
 

Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone

Article doi: 10.3791/59113
May 10th, 2019

Summary May 10th, 2019

Here, we present a detailed protocol to detect and quantify protein levels during craniofacial morphogenesis/pathogenesis by immunostaining using mouse craniofacial tissues as examples. In addition, we describe a method for preparation and cryosectioning of undecalcified hard tissues from young mice for immunostaining.

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