<em>Agrobacterium tumefaciens</em> and <em>Agrobacterium rhizogenes</em>-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Sandra Fern&#225;ndez-Pi&#241;&#225;n; Jennifer L&#243;pez; Iker Armendariz; Pau Boher; Merc&#232; Figueras; Olga Serra

doi:10.3791/59119

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Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

<em>Agrobacterium tumefaciens</em> and <em>Agrobacterium rhizogenes</em>-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

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Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

29,471 Views

•

08:31 min

•

March 29, 2019

DOI:

10.3791/59119-v

DOI:

10.3791/59119-v

•

08:31 min

March 29, 2019

•

29422 Views

Sandra Fernández-Piñán¹, Jennifer López¹, Iker Armendariz¹, Pau Boher¹, Mercè Figueras¹, Olga Serra¹

¹Department of Biology,Universitat de Girona

Summary

Here, we present two protocols to transform potato plants. The Agrobacterium tumefaciens transformation leads to a complete transgenic plant while the Agrobacterium rhizogenes produces transgenic hairy roots in a wild type shoot that can be self-propagated. We then detect promoter activity by GUS staining in the transformed roots.