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JoVE Journal Bioengineering
Meten mondiale cellulaire Matrix Metalloproteinase en metabole activiteit in 3D Hydrogels
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Meten mondiale cellulaire Matrix Metalloproteinase en metabole activiteit in 3D Hydrogels

Article DOI: 10.3791/59123-v • 07:39 min • January 22nd, 2019
January 22nd, 2019 •
1,2,3, 1,2
1Department of Biomedical Engineering, The Ohio State University, 2The Ohio State University Comprehensive Cancer Center, Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, 3Biomedical Technology Department, King Saud University

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Hier, een protocol wordt gepresenteerd voor het inkapselen van en het kweken van cellen in poly(ethylene glycol) (PEG) hydrogels matiemaatschappij met een fluorogenic matrix metalloproteinase (MMP)-afbreekbaar peptide. Cellulaire MMP en metabole activiteit wordt gemeten rechtstreeks vanaf de hydrogel culturen met behulp van een standaard microplate-lezer.

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Bioengineering kwestie 143 Hydrogels 3D cel inkapseling Matrix metalloproteinasen metabole activiteit melanoom fluorescentie
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