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JoVE Journal
Immunology and Infection

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Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells
 

Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells

Article doi: 10.3791/59124
March 5th, 2019

Summary March 5th, 2019

Here, we present a protocol to isolate dendritic cells from murine spleens by magnetic cell sorting and subsequent adoptive transfer into naïve mice. The model of high-salt activated dendritic cells was chosen to explain the step-by-step procedures of adoptive transfer and flow cytometry.

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