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JoVE Journal
Immunology and Infection

This content is Open Access.

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
 

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Article DOI: 10.3791/59126-v 12:09 min February 28th, 2019
February 28th, 2019

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Summary

This protocol describes the use of single chain MHC class I complexes to investigate molecular interactions in human CD8+ T cell activation: generation of engineered antigen presenting cells expressing single chain constructs, culture of human CD8+ T cell clone and T cell activation experiments.

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Single Chain MHC Technology Co-agonism Human CD8+ T Cell Activation Nonstimulatory Peptide MHC Complexes T Cell Responses Experimental System MHC Molecules Predetermined Peptides CTN Clone Hepatitis B Virus Mechanism Of Co-agonism T Cell Systems MHC Class I Molecules Signal Sequence Beta-2-microglobulin Linker MHC Heavy Chain E1A3 Peptide Hepatitis B Agonist Peptide Gag Peptide HIV Nonstimulatory Peptide
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