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Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
 

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Article doi: 10.3791/59128
February 12th, 2019

Summary February 12th, 2019

The protocol describes a reproducible method designed for use with cell culture supernatants to detect surface epitopes on small extracellular vesicles (EV). It utilizes specific EV immunoprecipitation using beads coupled with antibodies that recognize surface antigen CD9, CD63, and CD81. The method is optimized for downstream flow cytometry analysis.

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