Journal
/
Medicine
/
Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

DOI:
10.3791/59139-v

•

09:06 min

•

February 20, 2019

•

, , ,
1Heart, Lung, Blood and Vascular Medicine Institute,University of Pittsburgh, 2Robert M. Berne Cardiovascular Research Center,University of Virginia, 3Department of Biochemistry and Molecular Genetics,University of Virginia, 4Division of Cardiology,University of Pittsburgh School of Medicine

Chapters

  • 00:04Title
  • 00:36Brachiocephalic Artery (BCA) Harvest Preparation
  • 01:56BCA Exposure and Harvest
  • 03:05Immunofluorescent Staining
  • 05:04Confocal Microscopy and Single Cell Counting
  • 07:00Results: Representative Characterization of the Effects of IL-1beta Inhibition on the Cellular Composition of Artherosclerotic Lesions
  • 08:33Conclusion

Summary

Automatic Translation

Automatic Translation

We propose a standardized protocol to characterize the cellular composition of late-stage murine atherosclerotic lesions including systematic methods of animal dissection, tissue embedding, sectioning, staining, and analysis of brachiocephalic arteries from atheroprone smooth muscle cell lineage tracing mice.

Tags

Keywords: Quantitative AnalysisCellular CompositionAdvanced Atherosclerotic LesionsSmooth Muscle Cell Lineage-tracingImmunofluorescent StainingPhenotypic CharacterizationQualitative And Quantitative AnalysesCell TypesBrachiocephalic Artery HarvestGravity Perfusion SystemParaformaldehyde FixationCarotid ArterySubclavian ArteryAortic Arch

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59139?article_type=v

Myo-mechanical Analysis of Isolated Skeletal Muscle
check_url/59139?article_type=v

Quantitative Analysis of Chromatin Proteomes in Disease
check_url/59139?article_type=v

Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography
check_url/59139?article_type=v

Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus
check_url/59139?article_type=v

Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice
check_url/59139?article_type=v

Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease
check_url/59139?article_type=v

Ultrasound Based Assessment of Coronary Artery Flow and Coronary Flow Reserve Using the Pressure Overload Model in Mice
check_url/59139?article_type=v

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation
check_url/59139?article_type=v

Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition
check_url/59139?article_type=v

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Read Article