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JoVE Journal
Biochemistry

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Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
 

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

Article doi: 10.3791/59166
March 7th, 2019

Summary March 7th, 2019

Here we present a protocol to express, solubilize, and purify several eukaryotic borate transporters with homology to the SLC4 transporter family using yeast. We also describe a chemical cross-linking assay to assess the purified homomeric proteins for multimeric assembly. These protocols can be adapted for other challenging membrane proteins.

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