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JoVE Journal
Immunology and Infection

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Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking
 

Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking

Article doi: 10.3791/59167
March 7th, 2019

Summary March 7th, 2019

This study demonstrates the use of flow cytometry to detect reactive oxygen species (ROS) production resulting from activation of the FcγR. This method can be used to assess changes in the antimicrobial and redox signaling function of phagocytes in response to immune complexes, opsonized microorganisms, or direct FcγR cross-linking.

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