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Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
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Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

DOI:
10.3791/59169-v

•

09:21 min

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February 15, 2019

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, , , ,
1Southern Regional Research Center,USDA-ARS, Food and Feed Safety Research Unit, 2Department of Plant Pathology,University of Arkansas

Chapters

  • 00:04Title
  • 01:07Preparation of the Kernel Screening Assay (KSA)
  • 04:25Preparing the Kernels for Photography and Analysis
  • 06:07Results: Analysis of the Transgenic Maize
  • 08:23Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to analyze Aspergillus flavus growth and aflatoxin production in maize kernels expressing an antifungal protein.  Using a GFP-expressing A. flavus strain we monitored the infection and spread of the fungus in mature kernels in real time. The assay is rapid, reliable, and reproducible.

Tags

Keywords: Aspergillus FlavusAflatoxinMaizeTransgenicα-amylase InhibitorLablab PurpureusKernel Screening AssayGreen Fluorescent ProteinFungal InfectionAntifungal Properties

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