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Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
 

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Article DOI: 10.3791/59169-v 09:21 min February 15th, 2019
February 15th, 2019

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Summary

Here we present a protocol to analyze Aspergillus flavus growth and aflatoxin production in maize kernels expressing an antifungal protein.  Using a GFP-expressing A. flavus strain we monitored the infection and spread of the fungus in mature kernels in real time. The assay is rapid, reliable, and reproducible.

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Tags

Aspergillus Flavus Aflatoxin Production Transgenic Maize Alpha-amylase Inhibitor Lablab Purpureus Antifungal Protein Kernel Screening Assay Green Fluorescent Protein Infection Monitoring Resistance Evaluation Controlled Conditions Field Conditions Lab Assay Postdoc Biological Science Technicians KSA Caps Bioassay Tray Ethanol Spray Chromatography Paper Centrifuge Tube
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