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Remming van de groei van de Aspergillus flavus en aflatoxine productie in transgene maïs uiten van de α-amylase Inhibitor van Lablab purpureus L.
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Remming van de groei van de Aspergillus flavus en aflatoxine productie in transgene maïs uiten van de α-amylase Inhibitor van Lablab purpureus L.

Article DOI: 10.3791/59169-v • 09:21 min • February 15th, 2019
February 15th, 2019 •
1, 2, 1, 1, 1
1Southern Regional Research Center, USDA-ARS, Food and Feed Safety Research Unit, 2Department of Plant Pathology, University of Arkansas

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Hier presenteren we een protocol om Aspergillus flavus groei en productie van aflatoxine in maïs kernels uiting van een antischimmel eiwit te analyseren.  Met behulp van een stam GFP-uiten A. flavus bewaakt we de infectie en de verspreiding van de schimmel in volwassen kernels in real-time. De bepaling is snel, betrouwbaar en reproduceerbaar.

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Milieuwetenschappen kwestie 144 α-amylase-remmer aflatoxine Aspergillus flavus maïs kernel assay Lablab purpureus maïs transgene screening
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