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Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
DOI:

09:57 min

April 01, 2019

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Chapters

  • 00:04Title
  • 01:15Scaffold Manufacturing
  • 02:50Bone Marrow-Derived Mononuclear Cell (BM-MNC) Harvest
  • 04:40Cell Seeding
  • 05:21Graft Implantation
  • 08:28Results: Representative Basal Epithelial Cell and Stenotic Graft Region Analyses
  • 09:27Conclusion

Summary

Automatic Translation

Graft stenosis poses a critical obstacle in tissue engineered airway replacement. To investigate cellular mechanisms underlying stenosis, we utilize a murine model of tissue engineered tracheal replacement with seeded bone marrow mononuclear cells (BM-MNC). Here, we detail our protocol, including scaffold manufacturing, BM-MNC isolation, graft seeding, and implantation.

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