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Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

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09:57 min

April 01, 2019

DOI:

09:57 min
April 01, 2019

7273 Views
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Summary

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Graft stenosis poses a critical obstacle in tissue engineered airway replacement. To investigate cellular mechanisms underlying stenosis, we utilize a murine model of tissue engineered tracheal replacement with seeded bone marrow mononuclear cells (BM-MNC). Here, we detail our protocol, including scaffold manufacturing, BM-MNC isolation, graft seeding, and implantation.

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