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JoVE Journal
Bioengineering

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Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
 

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

Article doi: 10.3791/59173
April 1st, 2019 Usage Statistics

Summary April 1st, 2019

Graft stenosis poses a critical obstacle in tissue engineered airway replacement. To investigate cellular mechanisms underlying stenosis, we utilize a murine model of tissue engineered tracheal replacement with seeded bone marrow mononuclear cells (BM-MNC). Here, we detail our protocol, including scaffold manufacturing, BM-MNC isolation, graft seeding, and implantation.

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