Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

Matthew  G. Wiet; Sayali Dharmadhikari; Audrey White; Susan  D. Reynolds; Jed Johnson; Christopher  K. Breuer; Tendy Chiang

doi:10.3791/59173

Journal

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Bioengineering

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Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

7,292 Views

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09:57 min

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April 01, 2019

DOI:

10.3791/59173-v

DOI:

10.3791/59173-v

•

09:57 min

April 01, 2019

•

7273 Views

Matthew G. Wiet*^1,2, Sayali Dharmadhikari*^1,3, Audrey White^1,2, Susan D. Reynolds⁴, Jed Johnson⁵, Christopher K. Breuer^3,6, Tendy Chiang^1,3

¹Department of Otolaryngology Head & Neck Surgery,Nationwide Children’s Hospital ²The Ohio State University College of Medicine ³Center for Regenerative Medicine,Research Institute at Nationwide Children’s Hospital ⁴Center for Perinatal Research,Nationwide Children’s Hospital ⁵Nanofiber Solutions, Inc.⁶Department of Pediatric Surgery,Nationwide Children’s Hospital

Summary

Graft stenosis poses a critical obstacle in tissue engineered airway replacement. To investigate cellular mechanisms underlying stenosis, we utilize a murine model of tissue engineered tracheal replacement with seeded bone marrow mononuclear cells (BM-MNC). Here, we detail our protocol, including scaffold manufacturing, BM-MNC isolation, graft seeding, and implantation.