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A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

DOI:
10.3791/59194-v

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08:42 min

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April 10, 2019

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, , , ,
1Department of Pediatrics,Northwestern University, 2Division of Pediatric Surgery,Ann & Robert H. Lurie Children’s Hospital of Chicago

Chapters

  • 00:04Title
  • 01:25Crypt Isolation and Plating from Whole Tissue
  • 05:21Induction of Experimental NEC and Preparation for Paraffin Embedding
  • 07:22Results: Analysis of Enteroids
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

Enteroids are emerging as a novel model in the study of human disease. The protocol describes how to simulate an enteroid model of human necrotizing enterocolitis using lipopolysaccharide (LPS) treatment of enteroids generated from neonatal tissue. Collected enteroids demonstrate inflammatory changes akin to those seen in human necrotizing enterocolitis.

Tags

Keywords: Human Epithelial EnteroidsNecrotizing EnterocolitisIntestinal Stem CellsOrganoidsCell IsolationInflammationIntestinal EpitheliumCo-administrationLipopolysaccharide

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