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Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
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Cancer Research
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JoVE Journal Cancer Research
Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

DOI:
10.3791/59195-v

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07:29 min

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April 22, 2019

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, , , , ,
1Department of Radiation Oncology,University of Colorado Denver – Anschutz Medical Campus, 2Department of Anesthesiology,University of Colorado Denver – Anschutz Medical Campus, 3Division of Radiology,University of Colorado Denver – Anschutz Medical Campus

Chapters

  • 00:04Title
  • 00:32Tumor Cell Culture
  • 01:34Cell Injection
  • 02:41Mouse Monitoring and Tumor Harvesting
  • 03:31Tumor Processing
  • 04:51Results: Representative Mouse Squamous Cell Carcinoma Cell Tumor Profiling
  • 06:48Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a reproducible method for developing an orthotopic murine model of head and neck squamous cell carcinoma. Tumors demonstrate clinically relevant histopathological features of the disease, including necrosis, poor differentiation, nodal metastases, and immune infiltration. Tumor-bearing mice develop clinically relevant symptoms including dysphagia, jaw displacement, and weight loss.

Tags

Keywords: Intramucosal InoculationSquamous Cell CarcinomaTumor Immune ProfilingTreatment Response AssessmentHead And Neck CancerTumor MicroenvironmentTreatment ResistanceTumor Cell CultureTrypsinDMEM F12 MediumBasement Membrane MatrixTumor VolumeTumor Growth

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