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Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
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Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

DOI:
10.3791/59212-v

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05:44 min

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March 01, 2019

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, , , ,
1Department of Growth and Reproduction,Copenhagen University Hospital, 2International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC),University of Copenhagen

Chapters

  • 00:04Title
  • 00:40Motile Sperm Cell Purification
  • 01:28Sperm Cell Counting
  • 02:14Calcium-fluorimetry
  • 03:30Acrosome Reaction Measurement
  • 04:14Results: Representative Calcium-signaling and Acrosome Reaction Analyses
  • 05:01Conclusion

Summary

Automatic Translation

Automatic Translation

Here, two medium-throughput assays for assessment of effects on Ca2+-signaling and acrosome reaction in human sperm are described. These assays can be used to quickly and easily screen large amounts of compounds for effects on Ca2+-signaling and acrosome reaction in human sperm.

Tags

Keywords: Medium-throughput ScreeningCalcium SignalingAcrosome ReactionHuman SpermSperm Cell FunctionCompound TestingHTF MediumSperm Cell MotilitySperm Cell ConcentrationFluorescence Plate ReaderCalcium Concentration

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